svt2 mouse fibroblast cells (ATCC)
ATCC is a verified supplier 
ATCC manufactures this product 
94
Structured Review
ATCC
svt2 mouse fibroblast cells
Svt2 Mouse Fibroblast Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/svt2+mouse+fibroblast+cells/SV-T2/us09360484-3622-17-36
Average 94 stars, based on 8 article reviews
Svt2 Mouse Fibroblast Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/svt2+mouse+fibroblast+cells/SV-T2/us09360484-3622-17-36
Average 94 stars, based on 8 article reviews
svt2 mouse fibroblast cells - by Bioz Stars,
2026-09
94/100 stars
Images
Related Articles
Direct ELISA:Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to SVT2 mouse fibroblast cells stably expressing the FcRH of interest by FACS, were fused with mouse myeloma cells (X63.Ag8.653; American Type Culture Collection, Rockville, Md.) using a modified protocol analogous to one previously described (Kohler and Milstein, 1975; Hongo et al., 1995). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6 (SEQ ID NO: 68)) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Compositions and methods for the treatment of tumor of hematopoietic origin Article Snippet: Hollister, Calif.) or twenty Xenomiceä (Abgenix, Fremont, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to SVT2 mouse fibroblast cells stably expressing the FcRH of interest by FACS, were fused with mouse myeloma cells (X63.Ag8.653; American Type Culture Collection, Rockville, Md.) using a modified protocol analogous to one previously described (Kohler and Milstein, 1975; Hongo et al., 1995). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6 (SEQ ID NO: 68)) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Compositions and methods for the treatment of tumor of hematopoietic origin Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) or twenty Xenomiceä (Abgenix, Fremont, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Compositions and methods for the treatment of tumor of hematopoietic origin Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) or twenty Xenomiceä (Abgenix, Fremont, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Binding Assay:Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to SVT2 mouse fibroblast cells stably expressing the FcRH of interest by FACS, were fused with mouse myeloma cells (X63.Ag8.653; American Type Culture Collection, Rockville, Md.) using a modified protocol analogous to one previously described (Kohler and Milstein, 1975; Hongo et al., 1995). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6 (SEQ ID NO: 68)) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Compositions and methods for the treatment of tumor of hematopoietic origin Article Snippet: Hollister, Calif.) or twenty Xenomiceä (Abgenix, Fremont, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to SVT2 mouse fibroblast cells stably expressing the FcRH of interest by FACS, were fused with mouse myeloma cells (X63.Ag8.653; American Type Culture Collection, Rockville, Md.) using a modified protocol analogous to one previously described (Kohler and Milstein, 1975; Hongo et al., 1995). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6 (SEQ ID NO: 68)) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Compositions and methods for the treatment of tumor of hematopoietic origin Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) or twenty Xenomiceä (Abgenix, Fremont, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Compositions and methods for the treatment of tumor of hematopoietic origin Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) or twenty Xenomiceä (Abgenix, Fremont, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Stable Transfection:Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to SVT2 mouse fibroblast cells stably expressing the FcRH of interest by FACS, were fused with mouse myeloma cells (X63.Ag8.653; American Type Culture Collection, Rockville, Md.) using a modified protocol analogous to one previously described (Kohler and Milstein, 1975; Hongo et al., 1995). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6 (SEQ ID NO: 68)) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Compositions and methods for the treatment of tumor of hematopoietic origin Article Snippet: Hollister, Calif.) or twenty Xenomiceä (Abgenix, Fremont, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to SVT2 mouse fibroblast cells stably expressing the FcRH of interest by FACS, were fused with mouse myeloma cells (X63.Ag8.653; American Type Culture Collection, Rockville, Md.) using a modified protocol analogous to one previously described (Kohler and Milstein, 1975; Hongo et al., 1995). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6 (SEQ ID NO: 68)) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Compositions and methods for the treatment of tumor of hematopoietic origin Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) or twenty Xenomiceä (Abgenix, Fremont, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Compositions and methods for the treatment of tumor of hematopoietic origin Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) or twenty Xenomiceä (Abgenix, Fremont, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Expressing:Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to SVT2 mouse fibroblast cells stably expressing the FcRH of interest by FACS, were fused with mouse myeloma cells (X63.Ag8.653; American Type Culture Collection, Rockville, Md.) using a modified protocol analogous to one previously described (Kohler and Milstein, 1975; Hongo et al., 1995). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6 (SEQ ID NO: 68)) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Compositions and methods for the treatment of tumor of hematopoietic origin Article Snippet: Hollister, Calif.) or twenty Xenomiceä (Abgenix, Fremont, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to SVT2 mouse fibroblast cells stably expressing the FcRH of interest by FACS, were fused with mouse myeloma cells (X63.Ag8.653; American Type Culture Collection, Rockville, Md.) using a modified protocol analogous to one previously described (Kohler and Milstein, 1975; Hongo et al., 1995). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6 (SEQ ID NO: 68)) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Compositions and methods for the treatment of tumor of hematopoietic origin Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) or twenty Xenomiceä (Abgenix, Fremont, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Compositions and methods for the treatment of tumor of hematopoietic origin Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) or twenty Xenomiceä (Abgenix, Fremont, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to FACS:Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to SVT2 mouse fibroblast cells stably expressing the FcRH of interest by FACS, were fused with mouse myeloma cells (X63.Ag8.653; American Type Culture Collection, Rockville, Md.) using a modified protocol analogous to one previously described (Kohler and Milstein, 1975; Hongo et al., 1995). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6 (SEQ ID NO: 68)) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Compositions and methods for the treatment of tumor of hematopoietic origin Article Snippet: Hollister, Calif.) or twenty Xenomiceä (Abgenix, Fremont, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to SVT2 mouse fibroblast cells stably expressing the FcRH of interest by FACS, were fused with mouse myeloma cells (X63.Ag8.653; American Type Culture Collection, Rockville, Md.) using a modified protocol analogous to one previously described (Kohler and Milstein, 1975; Hongo et al., 1995). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6 (SEQ ID NO: 68)) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Compositions and methods for the treatment of tumor of hematopoietic origin Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) or twenty Xenomiceä (Abgenix, Fremont, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Compositions and methods for the treatment of tumor of hematopoietic origin Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) or twenty Xenomiceä (Abgenix, Fremont, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Modification:Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to SVT2 mouse fibroblast cells stably expressing the FcRH of interest by FACS, were fused with mouse myeloma cells (X63.Ag8.653; American Type Culture Collection, Rockville, Md.) using a modified protocol analogous to one previously described (Kohler and Milstein, 1975; Hongo et al., 1995). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6 (SEQ ID NO: 68)) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Compositions and methods for the treatment of tumor of hematopoietic origin Article Snippet: Hollister, Calif.) or twenty Xenomiceä (Abgenix, Fremont, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to SVT2 mouse fibroblast cells stably expressing the FcRH of interest by FACS, were fused with mouse myeloma cells (X63.Ag8.653; American Type Culture Collection, Rockville, Md.) using a modified protocol analogous to one previously described (Kohler and Milstein, 1975; Hongo et al., 1995). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6 (SEQ ID NO: 68)) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Compositions and methods for the treatment of tumor of hematopoietic origin Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) or twenty Xenomiceä (Abgenix, Fremont, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to Article Title: Compositions and methods for the treatment of tumor of hematopoietic origin Article Snippet: Ten Balb/c mice (Charles River Laboratories, Hollister, Calif.) or twenty Xenomiceä (Abgenix, Fremont, Calif.) were hyperimmunized with recombinant polyhistidine-tagged (HIS6) protein, in Ribi adjuvant (Ribi Immunochem Research, Inc., Hamilton, Mo.). .. B-cells from mice demonstrating high antibody titers against the immunogen by direct ELISA, and specific binding to |